Bca protein assay kit pdf
Web7 Apr 2024 · The pellet was resuspended in 300 µl 1 mM HEPES buffer, and the supernatant was saved. Protein concentration was determined with the BCA protein assay kit (Pierce). Western blot analysis of subcellular fractions and whole-cell homogenate of peri-infarct cortical tissue was performed as previously described. 8 WebIdentifying events that regulate the prenylation and localization of small GTPases will help define new strategies for therapeutic targeting of these proteins in disorders such as cancer, cardiovascular disease, and neurological deficits. Splice variants of the chaperone protein SmgGDS (encoded by RAP1GDS1) are known to regulate prenylation and trafficking of …
Bca protein assay kit pdf
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Web22 Mar 2024 · The protein samples were validated using BCA assay Kit (Thermo Fisher, USA), then denatured at 95°C for 10 minutes in loading buffer. 50μg protein samples were loaded and run on a 10% SDS–polyacrylamide gel, then transfer membrane and immunoblotted with primary antibodies against UCP-1 (1:1000, CST, USA) and beta actin … Web25 Oct 2024 · The BCA Protein Assay Kit (Thermo Fisher Scientific, 23227) was used to determine protein concentrations, according to the manufacturer’s instructions. Protein samples were resolved by SDS-PAGE and detected by immunoblotting with antibodies against c-Myc (CST, 13987), Bcl-XL (CST, 2764), p-STAT3 (CST, 9145), STAT3 (CST, …
Web8 Aug 2024 · Assay Protocol − BCA Assay can be performed in a microtiter plate format or test tube format. Microplate Procedure: 1. Add 25 µl of each BSA Standard and protein …
WebBCA Protein Quantification Kit is one of the most sensitive methods for protein assays. In an alkaline condition, Cu2+ can be reduced to Cu+ by protein and interacts with the … Web500-0112 Bio-Rad DC Protein Assay Kit II, includes contents of Reagents Package and bovine serum albumin standard 500-0116 Bio-Rad DC Protein Assay Reagents …
WebThe BCA assay Materials required depending on assay format used has many advantages over other protein determination but not provided techniques: • Spectrophotometer capable of accurately measuring …
Web2 days ago · These materials are not included in the kit, but will be required to successfully utilize this assay: Microplate reader capable of measuring absorbance at 450 or 600 nm. Method for determining protein concentration (BCA assay recommended). 3 Deionized water. Multi- and single-channel pipettes. Tubes for standard dilution. christin russmanWeb10 May 2024 · BCA Protein Assay Kit is a ready-to-use detergent- compatible Western blot related total protein analysis reagent used for the quick determination of total protein … christin rosefWeb28 Mar 2024 · BCA and Bradford protein assay in a miniaturized form is carried out on a polypropylene micro-test plate (PPµTP) using only 1 µl of the precious protein solution in the nanogram range without ... german language is also calledhttp://www.assay-protocol.com/biochemistry/BCA-assay.html german language is related to indian languageWebQuantiPro BCA Assay Kit Catalog Number QPBCA TECHNICAL BULLETIN Product Description Protein determination is one of the most common operations performed in … christin sampWebUP40840B BC Assay Protein Quantitation Kit, sufficient reagents for 125 tests/tube or 1 250 microplate assays Contains: UP95424B, reagent A, 250mL (.Z) ... known as the BCA method. Our improved formulation, the BC Assay, provides significant benefits over existing colorimetric assays, namely sensitivity, reagent stability, ... german language is also known asWeb23250 Pierce BCA Protein Assay Kit . −. Reducing Agent Compatible, sufficient reagents to assay 250 samples or standards of 25µL each Assay Working Range: 125 … german language jobs near me