WebOct 28, 2024 · To investigate the mechanism of the combination effect of AZD1775 and IR on cell proliferation, we investigated the downstream targets of Wee1 kinase in SiHa cells. After 24 h from IR with 4 or 6 Gy, western blotting was performed to investigate the effects of AZD1775 on phosphorylated-Cdc2 (pCdc2) and Cyclin B1. Webkinase Cdc2 antibody; MPF antibody; p33 protein kinase antibody; p33(CDK2) antibody; see all. Images. Western blot - Anti-Cdk2 antibody (ab235941) All lanes : Anti-Cdk2 antibody (ab235941) at 1/1000 dilution Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Anti-Cdk1/Cdc2 (PSTAIR) Antibody Upstate®, from rabbit - Sigma-Aldrich
WebSynthetic peptide corresponding to human cdc2 phosphorylated at threonine 14. Formulation. Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) Preparation. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. Concentration. WebBackground. The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin … flights from smf to mexico city
Recombinant Anti-Cdk2 antibody [E304] (ab32147) - Abcam
WebWestern blot analysis of Phospho-CDC2 (Thr14) in Various whole cell extracts (30 µg). Samples were separated with 12% SDS-PAGE and incubated in Phospho-CDC2 (Thr14) polyclonal antibody (Product # PA5-117399) using a dilution of 1:500, followed by HRP-conjugated anti-rabbit IgG. WebMar 30, 2024 · Affinity-purified antibody generated against the C-terminal 13 amino acids of Plk specifically recognizes a single polypeptide of 66 kDa in MELC, NIH 3T3, and HeLa cellular extracts. The expression levels of both poly(A) ... Concurrent with cyclin B-associated cdc2 kinase activity, Plk kinase activity sharply peaks at the onset of mitosis. ... WebRabbit anti Human CDC2 antibody recognizes CDC2, also known as CDK1, CDC28A, P34CDC2, p34 protein kinase or cyclin-dependent kinase 1. The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), … cherry corner computer desks