Cycleave rt-pcr core kit
WebThe assay was performed using a commercially available kit, e in a reaction volume of 25 µL containing deoxyribonucleotide triphosphates (300 µM), MgCl 2 (5 mM), 6-carboxyfluorescein (FAM)- and hexachlorofluorescein (HEX)-labeled probes (200 nM each), primers (400 nM each), Tli RNaseH II (100 units), Ex Taq HS (1.25 units), and the DNA … WebThe Taq PCR Core Kit provides all the components required to set up a PCR and can be used for a multitude of PCR-based applications. The …
Cycleave rt-pcr core kit
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WebTotal volume (30 µL) of the PCR mixture included 20 pmol of each primer, 25 pmol of each probe, and reagents from the Cycleave PCR Core kit (Takara Bio, Shiga, Japan). Real-time PCR was carried out for 45 cycles as follows: 95°C for 5 seconds, 55°C for 15 seconds and 72°C for 20 seconds. WebAug 15, 2013 · PDFPRINTCPT Code: 86140 Order Code: C1323 ABN Requirement: No Synonyms: C-Reactive Protein Specimen: Serum Volume: 1.0 mL Minimum Volume: …
WebPCR Core Kit For standard and specialized PCR applications, includes dNTP mix QIAGEN PCR Buffer for minimal optimization Additional ready-to-load PCR buffer for faster handling Q-Solution for amplification of GC-rich templates Choice of formats for convenience and ease of handling WebCycleavePCR Core Kit and Primer Sets . CycleavePCR Core Kit and primer setThe CycleavePCR Core Kit is designed exclusively for real-time PCR with cycling probe detection. This kit combines the quick and quantitative nature of real-time ... The store will not work correctly in the case when cookies are disabled. ...
WebPremix Ex Taq TM(Perfect Real Time)(TaKaRa Code RR039A/B)SYBR Premix Ex Taq TM(Perfect Real Time)(TaKaRa Code RR041A/B)CycleavePCR Core Kit(TaKaRa Code CY501) RT-PCR Kit(Perfect Real Time)(TaKaRa Code RR043A)SYBR RT-PCR Kit(Perfect Real Time)(TaKaRa Code RR045A)Cycleave RT-PCR Core Kit(TaKaRa Code CY502) … WebTaKaRa cycleave pcr core kit Cycleave Pcr Core Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
WebApr 1, 2014 · The PCR reaction system was prepared as per the instructions of the Cycleave PCR® Core Kit (TaKaRa, Dalian, China) containing 1 μL probe with PMPS, PMP2063, and PMP2064 at concentrations of 6, 10, and 10 μmol/L, respectively, and 1 μL DNA template. PCR cycling was performed under the conditions optimized by the …
WebTaq PCR Core Kit is provided in a complete kit format comprising, Taq DNA Polymerase, the unique QIAGEN PCR Buffer that minimizes the requirement for optimization, as well … shark tooth found in myrtle beachWebCycleavePCR Core Kit and Primer Sets . CycleavePCR Core Kit and primer setThe CycleavePCR Core Kit is designed exclusively for real-time PCR with cycling probe … shark tooth hat bandWebDescription: This product is one of Cycleave RT-PCR Detection Set series, each of which is a primer/probe set designed to detect the particular housekeeping gene by real time RT … shark tooth fossil ageWebSearch Products by Name, Cat #, CAS #, or Keyword. ×. Login / Register sharktooth hillWebReal-time PCR. Real-time PCR kits. Probe-based qPCR mixes. 5X One-Step PrimeScript III RT-qPCR; One-Step PrimeScript RT-PCR Kit (Perfect Real Time) One Step PrimeScript … shark tooth heart rhythmWebOct 4, 2006 · RT-PCR conditions were as follows: 1 cycle of 50°C for 30 minutes and 95°C for 15 minutes, 40 cycles of 94°C for 50 seconds, 62°C for 50 seconds, and 72°C for 1 … population number in ukraineWebJul 1, 2006 · This assay was performed using a cycleave PCR core kit (TAKARA, Co., Ltd., Ohtsu, Japan), and sequences of the primer set and the probes were as follows: PCR forward primer for L858R, 5′-AGGAACGTACTGGTGAAAAC-3′; PCR reverse primer for L858R, 5′-TCCCTGGTGTCAGGAAAATG-3′; wild-type probe for L858R, 5′ FAM-CCA U … population number of the world