WebJun 7, 2024 · The lysine-reactive N-hydroxysuccinimide ester reagent disuccinimidylsuberate (DSS) (Thermo Scientific Waltham, MA USA) was used according to manufacturer's manual. Briefly, cells were washed three times in ice-cold PBS and then suspended at a concentration of 25 × 10 6 cells/ml, DSS was added at a final … WebThermo Fisher Scientific
Thermo Scientific DSS (disuccinimidyl suberate), No …
WebThermo Scientific™ Pierce Co-Immunoprecipitation Kit provides all the components necessary to perform a properly controlled co-IP experiment and obtain purified products without antibody contamination. The Co-Immunoprecipitation (Co-IP) Kit enables isolation of native protein complexes from a lysate or other complex mixture by directly ... WebFor single-junction Ag/AgCl electrodes, Calcium Combination ISEs, inner chamber of many double-junction electrodes Shop Filling Solution: 4M KCl saturated with AgCl at Fishersci.com html compiler 绿色
Xlink Mapping and AnalySis (XMAS) - Smooth Integrative …
WebDextran Sulfate Sodium Salt, Thermo Scientific™ Click to view available options Quantity: 10 g 50 g 100 g Packaging: Plastic bottle CAS: 9011-18-1: Molecular Formula: C9H11NO2: Molecular Weight (g/mol) 165.192: MDL Number: MFCD00081551: InChI Key: BLFLLBZGZJTVJG-UHFFFAOYSA-N ... WebApr 29, 2024 · DSS crosslinking was performed using 1 mM crosslinker concentration followed by 30-min incubation at 37°C. EDC crosslinking was performed with the same crosslinker concentration and incubation time, in the presence of 2 mM Sulfo-NHS. All chemicals were purchased from Sigma-Aldrich, unless mentioned otherwise. WebJun 17, 2024 · To determine whether the glutaraldehyde cross-linker resulted in protein aggregation, disuccinimidyl suberate (DSS; Thermo Fisher Scientific) cross-linking was also tested. Purified, wild-type RABV-G ectodomains were incubated with increasing concentrations of DSS according to the manufacturer’s protocol, covering the entire … hockley bars nottingham