Intron gus
WebMay 11, 2013 · This study was conducted to obtain intron-GUS gene-transferred plants by using somatic embryos (including embryogenic calluses) derived from 2 in vitro root … WebThe ability to target marker proteins to specific subcellular compartments is a powerful research tool to study the structure and development of organelles. Here transit sequences from nuclear-encoded, plastid proteins, namely rice FtsZ, maize non-photosynthetic ferredoxin III (FdIII) and the small subunit of RubisCO were used to target a modified …
Intron gus
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WebNov 12, 2010 · The nta-1 allele has a 20–base pair deletion at the exon 13/intron 13 boundary, ... The expression pattern of NTA was examined with translational fusions of the NTA genomic sequence to genes encoding GUS and green fluorescent protein (GFP) under control of the native NTA 5′-regulatory sequences. WebDec 13, 2013 · Intron effect on GUS transgene expression in transgenic rice lines 43. Constructs used in the study pRESQ4: rubi3 promoter—5’UTR exon1 (67 bp)----5’UTR intron---the GUS coding sequence pPSRG30: same as pRESQ4 (except 5’UTR intron) 44. Southern hybridization and real time PCR 45. GUS histochemical assay 46.
Webincluding the intron downstream of the pOp6 promoter and the omega leader sequence of tobacco mosaic virus to enhance translation. The coding sequence was followed by the ... the GUS gene in antisense orientation as a spacer, the second loxP site and the vYFPer reporter gene, encoding an ER-localized, cell autonomous form of YFP. Web120001-130000: ... Definition from Wiktionary, the free dictionary
WebGUS et au gène codant pour la saporine, une protéine cytotoxique. Suite à la transformation ... intron Recent studies have determined that a sequence of 1653 bp from the second intron was required for normal AG activity (Deyholos et … WebSalt-induced repression of MsPDH1 promoter linked to the GUS reporter gene confirmed that the decline in MsPDH transcript levels was due ... Medicago sativa roots. MsP5CS-1 (2.6 kb) has an open reading frame of 717 amino acids, as well as a non-spliced intron at a position corresponding to the evolutionary fusion point of the bacterial ...
WebPlasmid pZY102 from Dr. Zhanyuan Zhang's lab contains the inserts bar and GUS and is published in Plant Cell Rep. 2004 Feb;22(7):478-82. Epub 2003 Sep 30. This plasmid is available through Addgene. ... This plasmid was derived from pZY101 by inserting a GUS-intron reporter gene cassette into multiple cloning sites.
Webglucuronidase (GUS) reporter gene expression in the enhancer trap line is observed specifically in all cell layers of the valve tissue, but not in the replum, the septum or the seeds, and faithfully mimics RNA in situ hybridization data reported previously. The lack of coordinated growth of the buehler\u0027s buy low adWebEither of the first two introns of the Arabidopsis tryptophan pathway gene PAT1 elevates mRNA accumulation from a PAT1:β-glucuronidase (GUS) fusion roughly 5-fold without … budweiser ads super bowlWebApr 26, 2024 · The pFHI-GUSi binary vector carries the nptII gene and the intron version of the β-glucuronidase gene (intron-GUS), which prevents bacterial expression of the enzyme. Two days prior to co-cultivation with the plant cells, the Agro with pFHI-GUSi plasmid was grown on semi-solid YEP medium supplemented with 50 mg/L rifampicin, 100 mg/L … buehlers alcoholWebJul 6, 2024 · In both species, expression of 35S pro:GUS intron was however weaker than that of the 35S pro:GUS construct, likely due to the presence of an enhanced 35S … buell hawkinsWebb-glucuronidase (gus) activity on the 3rd and 4th day of co-cultivation of the infected Immature Zygotic Embryos (IZEs) and embryogenic callus. Transient gus expression was influenced by the co-cultivation period, maize geno-type and Agrobacterium strain. The expression was highest after the 3rd day of co-culture compared to the 4th day buehler\\u0027s in wadsworthWeb开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 budweiser clydesdales at busch stadium 2022WebJul 1, 2004 · The Intron cassette was designed in such a way that barnase and GUS genes are transcribed in opposite directions with respect to one another (Fig. 1). Barnase … budweiser metal ice bucket