Thiol phosphoramidite
WebA new phosphoramidite derivative and a new solid support incorporating the protected maleimide moiety were prepared for this purpose. In all cases maleimide deprotection (retro-Diels–Alder reaction) followed by reaction with thiol-containing compounds afforded the target conjugate. This article is part of the themed collection ... A phosphoramidite (RO)2PNR2 is a monoamide of a phosphite diester. The key feature of phosphoramidites is their markedly high reactivity towards nucleophiles catalyzed by weak acids e.c., triethylammonium chloride or 1H-tetrazole. In these reactions, the incoming nucleophile replaces the NR2 moiety.
Thiol phosphoramidite
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WebTetrahydro-thienopyridines antiaggregant prodrugs belong to a new class of “covalent drugs.” [104] These antithromboplastic compounds must be activated to an active thiol metabolite, which inactivates the ADP platelet receptor P2Y12.This is a four-step reaction [105,106].The first step of ticlopidine and clopidogrel activation is a CYP-dependent … WebXanthen-Phosphoramidite und -Trägermaterialien (außer FAM) ... Thiol Amine Maleimide Phosphoramidite CPG-Trägermaterialien Carbonsäuren Triphosphate Dimethyl Halogenide Cellular components . Cell membrane ...
WebNov 28, 2011 · Modified deoxy- and ribo-nucleoside triphosphates are chemically synthesized in multiple steps due to the protection and deprotection of the nucleoside functionalities. To conveniently synthesize the S-modified triphosphates for enzymatically preparing phosphorothioate DNAs and RNAs (PS-DNA and PS-RNA) as potential … WebThe synthesis of the 5′-Se-derivatized thymidine and phosphoramidite is reported, and the Se-phosphoramidite was incorporated into DNA. Because of its high nucleophilicity, this 5′-Se-moiety permits convenient postsynthetic modification of oligonucleotides. The proof of principle was successfully demonstrated by incorporating fluorescein into DNA. It was …
WebJul 26, 2024 · Thiol-modifier phosphoramidites. An oligonucleotide with thiol groups is an important intermediate in the production of fluorescent or other similar oligonucleotide conjugates. Applications for thiol modifiers are quite similar to amino-modifiers. Thiol groups containing phosphoramidites are used for the introduction of thiol groups into ... Web5'-Thiol C-6 Disulfide Modifier CED phosphoramidite. Part No. CLP-8506. FW 769.050. Formula C42H61N2O5PS2. Resources & Protocols View Thiol Modifier Amidites and Supports Application Notes >. View Chromophores & Ligands Application Notes >.
WebDusQ 21 is a dark quencher used as an acceptor in FRET applications, it has absorption maximum 656 nm, and can be used in multiplex assays due to the lack of secondary fluorescence. It is a quencher of choice for pairing with fluorescent dyes that emit in the red and near-infrared part of the range. DusQ 21 NHS ester derivative is used for ...
WebUS headquarters: +1 510 931 4711 Sales Inquiry Ext. 303 / Shipping Logistics Ext. 304 / General Ext. 305 mailbox Primary contact: [email protected] spai softwareWebAlkyne dT phosphoramidite,This reagent is designed for the synthesis of oligonucleotides bearing triple bond (alkyne) in the middle of the chain. Alkyne is attached to deoxyuridine fragment, and it does not interfere with the hybridization to the complementary strand. teamworks workdayWebThe thiol is init … We describe the synthesis of protected phosphoramidites of deoxyriboguanosine and guanosine derivatives containing a thiopropyl tether at the guanine N2 (7a,b) for site-specific crosslinking from the minor groove of either DNA or RNA to a thiol of a protein or another nucleic acid. spaital awarness gold lock onlineWebOur CAL Fluor™ and Quasar™ dye phosphoramidites are fluorophores with emission spectra that span the visible and near infrared spectrum, making them ideal for multiplexed real time qPCR assays. Biosearch Technologies also offers modifications such as Biotin, Amine, Thiol, Spacers as well as other popular fluorescent dye phosphoramidites. teamworks xavierWebApr 15, 2011 · The synthesis of the 5′-Se-derivatized thymidine and phosphoramidite is reported, and the Se-phosphoramidite was incorporated into DNA. Because of its high nucleophilicity, this 5′-Se-moiety permits convenient post-synthetic modification of oligonucleotides. ... (such as an amino or thiol group 5) during the solid-phase synthesis, … spa islip foot and body worksWebApr 21, 2005 · For studies of RNA structure, folding, and catalysis, site-specific modifications are typically introduced by solid-phase synthesis of RNA oligonucleotides using nucleoside phosphoramidites. Here, we report the preparation of two complete series of RNA nucleoside phosphoramidites; each has an appropriately protected amine or thiol … teamworks wmWebThiol SS Dipod (DTPA) Dithiol Phosphoramidite (DTPA) is a disulfide-containing modifier designed to functionalize synthetic DNA or RNA with multiple thiol groups and can be incorporated at any position of the oligonucleotide. Each DTPA addition leads to two thiol groups. This modifier was designed for optimal tethering of oligonucleotides to a ... teamworks workstation wont update